After cleavage with T4-3C protease, the expression product produced several protein bands that could react with FMD positive immune serum. 表达产物经T4-3C蛋白酶作用,裂解后可产生多种蛋白。出现与抗口蹄疫血清反应的蛋白条带。
T1 was released after thrombin cleavage of the fusion protein. GST protein was removed with affinity chromatography, and after that T1 was purified by ion exchange chromatography. The purity of the final product was over 90%. 融合蛋白经凝血酶裂解后,释放出Tα1单体,先经亲和层析除去酶切后产生的谷胱甘肽-硫-转移酶(GST),然后通过离子交换纯化Tα1,纯度大于90%。
Moreover, the large cleavage product of Pref-1 can act in an endocrine manner to inhibit adipocyte differentiation in vivo. 大的可溶性Pref-1片段能以内分泌的方式抑制体内脂肪细胞的分化。
Total selectivity of C-C cleavage product for monosaccharide and disaccharide are high, but the polysaccharide are low. 单糖和二糖的C-C断裂产物的总选择性较高,而多糖则很低。
When the reaction time increased, the glucose conversion increased, the total selectivity of C-C cleavage oxidation increased too, if the time is too long, the selectivity of product reduced, this may be caused by the decarboxylation of products. 反应时间增加,转化率增大,C-C键断裂氧化产物总选择性增加,反应时间过长选择性降低。
As the amount of glucose increased, the conversion gradually decreased, the selectivity of C-H cleavage product increased at first then decreased and keep stable finally, total selectivity of C-C cleavage product keep in a stable level. 葡萄糖量增加,转化率降低,葡萄糖酸的选择性先增加后降低并趋于稳定,C-C断裂产物的总选择性基本不变。
Using molecular beacon as the template and the signal molecule for ribozyme cleavage product, the information of cleavage product has been directly converted into fluorescent signal during RNA/ DNA ligation process. 本论文利用分子信标作为核酶切割产物的连接模板和检测分子,在RNA/DNA核酸杂合体连接过程中,核酶切割产物的信息被实时转换为荧光信号。
Ultrasensitive detection method for restriction enzyme cleavage product using molecular beacon. 建立了限制性内切酶切割产物的超灵敏检测方法。